THE WHEAT LEAF PHOSPHATASES
نویسندگان
چکیده
منابع مشابه
The wheat leaf phosphatases. IV. The effect of metal ions on the acid phosphatase activity of dialyzed juice.
In Papers I and III on the wheat leaf phosphatases the effects of inhibitors (1) and heat treatment (2) on the enzyme activity have been reported. To define the substrate specificities of the enzymes involved, purification involving dialysis of the preparations will be necessary. Thus it is essential to know whether dialysis will cause loss of activity and, if so, what metal ions will restore t...
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Two protein phosphatases (enzymes I and II) were extensively purified from wheat embryo by a procedure involving chromatography on DEAE-cellulose, phenyl-Sepharose CL-4B, DEAE-Sephacel and Ultrogel AcA 44. Preparations of enzyme I (Mr 197,000) are heterogeneous. Preparations of enzyme II (Mr 35,000) contain only one major polypeptide (Mr 17,500), which exactly co-purifies with protein phosphata...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1958
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)70556-5